Application

Anti-HA-Fluorescein, High Affinity has been used in immunofluorescence labeling of chinese hamster ovary live cells and in immunocytochemistry of neuron cell culture.

General description

Anti-HA-Fluorescein, High Affinity is a monoclonal antibody for the highly sensitive detection of HA-tagged recombinant proteins, Fab fragments, conjugated to fluorescein.Anti-HA-Fluorescein, High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the human hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".ContentsLyophilizate, stabilized

Immunogen

Amino acids 98 to 106 from the human influenza virus hemagglutinin protein

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Stabilizers: proteinous stabilizersWorking concentration: Flow cytometry: 1 to 5 µg/mlImmunofluorescence: 1 to 5 µg/mlWorking concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline.Storage conditions (working solution): Reconstituted antibody is stable at 2 to 8 °C for two months. Alternatively store aliquots at -15 to -25 °C for up to six months.Note: Avoid thawing and repeated freezing.

Quality

The Anti-HA-Fluorescein, High Affinity antibody is function tested by flow cytometry analysis of a HA-tagged fusion protein

Reconstitution

Add 0.5 ml double-distilled water to a final concentration of 50 µg/ml. Rehydrate for 10 minutes prior to use.

Specificity

Anti-HA-Fluorescein, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).